Diacetylcurcumin: a novel strategy against Enterococcus faecalis biofilm in root canal disinfection.

Aim: We evaluated Diacetylcurcumin (DAC), a derivative of curcumin, for its antibacterial and antibiofilm properties against Enterococcus faecalis. Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration were determined, along with antibiofilm potential and toxicity in Galleria mellonella. Additionally, in silico computational analysis was performed to understand its mechanisms of action. Results & conclusion: DAC demonstrated significant antibacterial effects, with MIC and MBC values of 15.6 and 31.25 µg/ml, respectively, and reduced biofilm formation. A synergistic effect, reducing biofilm by 77%, was observed when combined with calcium hydroxide. G. mellonella toxicity tests confirmed DAC's safety at tested concentrations, suggesting its potential for use in root canal disinfection products.

Diacetylcurcumin (DAC) comes from turmeric, a natural spice often used in food. DAC may have the ability to fight germs, including the bacteria Enterococcus faecalis. We tested DAC's ability to kill E. faecalis and stopping the formation of films of the bacteria. We found that a small amount of DAC did kill E. faecalis. When used with calcium hydroxide, DAC works even better to reduce the formation of bacterial films by 77%. DAC is safe to be used on teeth, so may be a useful ingredient for preserving mouth health.

Evaluation of efficacy of new chalcone-based endodontic irrigant against dual biofilm Enterococcus faecalis and Candida albicans: a study in vitro.

The aim of this research was to develop a chalcone-based endodontic irrigant for cleaning and disinfecting the root canal. Minimal inhibitory concentration (MIC) experiments in C. albicans and E. faecalis strains with different aminochalcones (AM) were carried out, and the compound that presented the best activity against both pathogens was chosen. The formulation of an endodontic irrigant was elaborated, tested in mono and dual specie biofilms. Disks were sterilized and then incubated with E. faecalis, C. albicans and E. faecalis and C. albicans mixed for 72 h for biofilm maturation. After contamination, samples were divided in 4 experimental groups and 2 positive control group as follows: Group1: Irrigant; Group2: Irrigant + AM-38; Group3: Chlorhexidine 2% (positive control) and, Group 4: 1.0% sodium hypochlorite (positive control). The samples were analyzed by CFU/ml count. The sample was taken to sonicador to remove the cells and then plated. The toxicity was determined in vitro with human gingival fibroblast cells (HGF) and in vivo using the Galleria mellonella model. Formulation showed antimicrobial activity, with MIC on C. albicans 15.6 and E. faecalis 7.8 µg/ml. Treatment with formulation in concentration 156 µg/ml significantly reduced mono or dual species biofilm formation and viability (p < 0.05). The results were significant against C. albicans and E. faecalis and did not show toxicity in cells and G. mellonella. In general, the formulation showed effective antibiofilm activity, significantly reducing microorganisms, opening paths in search of new endodontic irrigants.

Biological properties of Schinus terebinthifolia Raddi essential oil

Abstract Schinus terebinthifolia Raddi green fruits essential oil (EO) was evaluated regarding its phytochemical profile, antimicrobial and cytotoxic activities, and toxicity. Gas chromatography with mass spectrometry was applied to identify its constituents, thereafter the minimum inhibitory concentration, minimum bactericidal and fungicidal concentrations, and its antibiofilm activity were evaluated. The EO cytotoxicity was assessed in tumor and non-tumor human cells, and in vivo toxicity was evaluated in a Galleria mellonella model. The major constituents of S. terebinthifolia EO were alpha-phellandrene and beta-phellandrene. The EO had a weak activity against all strains of Candida albicans (MIC 1000µg/mL) and had no activity against non-albicans strains, bacteria, and C. albicans biofilm. Cytostatic activity against all tumor cell lines was shown. Additionally, cell viability remained at EO concentrations up to 62.5 µg/mL. At 16 mg/mL, 50% hemolysis was observed, and it had low toxicity in vivo. Overall, the S. terebinthifolia EO was characterized by low antimicrobial and antibiofilm activities, with no evidence of toxicity to human tumor and non-tumor cells

Phytochemical composition, antifungal activity, in vitro and in vivo toxicity of Syzygium cumini (L) Skeels leaves extract / Composición fitoquímica, actividad antifúngica, toxicidad in vitro e in vivo del extracto de hojas de Syzygium cumini (L) Skeels

This study determined phytochemical composition, antifungal activity and toxicity in vitro and in vivo of Syzygium cumini leaves extract (Sc). Thus, was characterized by gas chromatography coupled to mass spectrometry and submitted to determination of Minimum Inhibitory (MIC) and Fungicidal concentrations (MFC) on reference and clinical strains of Candida spp. and by growth kinetics assays. Toxicity was verified using in vitro assays of hemolysis, osmotic fragility, oxidant and antioxidant activity in human erythrocytes and by in vivo acute systemic toxicity in Galleria mellonella larvae. Fourteen different compounds were identified in Sc, which showed antifungal activity (MIC between 31.25-125µg/mL) with fungistatic effect on Candida. At antifungal concentrations, it demonstrated low cytotoxicity, antioxidant activity and neglible in vivotoxicity. Thus, Sc demonstrated a promising antifungal potential, with low toxicity, indicating that this extract can be a safe and effective alternative antifungal agent.

Este estudio determinó la composición fitoquímica, la actividad antifúngica y la toxicidad in vitro e in vivo del extracto de hojas de Syzygium cumini (Sc). Así, se caracterizó mediante cromatografía de gases acoplada a espectrometría de masas y se sometió a determinación de Concentraciones Mínimas Inhibitorias (CMI) y Fungicidas (MFC) sobre cepas de referencia y clínicas de Candida spp. y mediante ensayos de cinética de crecimiento. La toxicidad se verificó mediante ensayos in vitro de hemólisis, fragilidad osmótica, actividad oxidante y antioxidante en eritrocitos humanos y por toxicidad sistémica aguda in vivo en larvas de Galleria mellonella. Se identificaron catorce compuestos diferentes en Sc, que mostraron actividad antifúngica (CMI entre 31.25-125 µg/mL) con efecto fungistático sobre Candida. En concentraciones antifúngicas, demostró baja citotoxicidad, actividad antioxidante y toxicidad in vivo insignificante. Por lo tanto, Sc demostró un potencial antifúngico prometedor, con baja toxicidad, lo que indica que este extracto puede ser un agente antifúngico alternativo seguro y eficaz.

Anti-inflammatory and anti-Candida Effects of Brazilian Organic Propolis, a Promising Source of Bioactive Molecules and Functional Food.

Brazilian organic propolis (BOP) is an unexplored Brazilian propolis that is produced organically and certified according to international legislation. Our results showed that BOP has strong anti-inflammatory effects and acts by reducing nuclear factor κB activation, tumor necrosis factor α release, and neutrophil migration. In addition, BOP6 exhibited antifungal activity on planktonic and biofilm cultures of Candida albicans, Candida glabrata, Candida tropicalis, Candida krusei, and Candida parapsisolis and reduced in vitro yeast cell adhesion to human keratinocytes at sub-inhibitory concentrations. BOP demonstrated significantly low toxicity in Galleria melonella larvae at antifungal doses. Lastly, a chemical analysis revealed the presence of caffeoyltartaric acid, 3,4-dicaffeoylquinic acid, quercetin, and gibberellins A7, A9, and A20, which may be responsible for the biological properties observed. Thus, our data indicate that BOP is a promising source of anti-inflammatory and antifungal molecules that may be used as a functional food.

Anadenanthera Colubrina vell Brenan: anti-Candida and antibiofilm activities, toxicity and therapeutical action.

We evaluated the antifungal and antibiofilm potential of the hydroalcoholic extract of bark from Anadenanthera colubrina (vell.) Brenan, known as Angico, against Candida spp. Antifungal activity was evaluated using the microdilution technique through the Minimum Inhibitory and Fungicide Concentrations (MIC and MFC). The antibiofilm potential was tested in mature biofilms formed by Candida species and analyzed through the counting of CFU/mL and scanning electron micrograph (SEM). In vivo toxicity and therapeutic action was evaluated in the Galleria mellonella model. The treatment with the extract, in low doses, was able to reduce the growth of planktonic cells of Candida species. MIC values range between 19.5 and 39 µg/mL and MFC values range between 79 and 625 µg/mL. In addition was able to reduce the number of CFU/mL in biofilms and to cause structural alteration and cellular destruction, observed via SEM. A. colubrina showed low toxicity in the in vivo assay, having not affected the viability of the larvae at doses below 100mg/kg and high potential in the treatment of C. albicans infection. Considering its high antifungal potential, its low toxicity and potential to treatment of infections in in vivo model, A. colubrina extract is a strong candidate for development of a new agent for the treatment of oral candidiasis.

In silico approaches for screening molecular targets in Candida albicans: A proteomic insight into drug discovery and development.

Candida species are opportunistic pathogens which can cause conditions ranging from simple mucocutaneous infections to fungemia and death in immunosuppressed and hospitalized patients. Candida albicans is considered to be the species mostly associated with fungal infections in humans and, therefore, the mostly studied yeast. This microorganism has survival and virulence factors which, allied to a decreased host immunity response, make infection more difficult to control. Today, the current limited antifungal arsenal and a dramatic increase in fungal resistance have driven the need for the synthesis of drugs with novel mechanisms of action. However, the development of a new drug from discovery to marketing takes a long time and is highly costly. The objective of this review is to show that with advances in biotechnology and biofinformatics, in silico tools such as molecular docking can optimize such a timeline and reduce costs, while contributing to the design and development of targeted drugs. Here we highlight the most promising protein targets in Candida albicans for the development of drugs with new mechanisms of action.

Os produtos naturais são uma alternativa para o tratamento da candidose oral? Uma revisão de ensaios clínicos / Are natural products an alternative for the treatment of oral candidosis? A review of clinical trials / ¿Los productos naturales son una alternativa para el tratamiento de la candidiasis oral? Una revisión de los ensayos clínicos

Introdução: a candidose oral é uma doença oportunista que acomete principalmente pacientes imunossuprimidos e usuários de prótese dentária total. Apesar da eficácia terapêutica dos antifúngicos utilizados atualmente para o tratamento desta doença, essas drogas apresentam muitos efeitos adversos relacionados à dose. Além disso, tem-se observado um aumento da resistência microbiana para esses agentes. Baseado nisso, uma fonte de busca promissora de princípios ativos alternativos para o tratamento da candidose oral são os produtos naturais. Objetivo: revisar a literatura sobre ensaios clínicos com produtos naturais para o tratamento da candidose oral, a fim de responder se existe evidência que os produtos naturais podem ser utilizados como tratamento alternativo para esta doença. Material e método: foi realizada uma síntese de todos os ensaios clínicos com produtos naturais para tratamento da candidose indexados banco de dados Pubmed. Resultado: seguindo os critérios de inclusão e exclusão entraram nesta revisão 6 estudos. Os estudos avaliaram a eficácia dos produtos naturais quanto aos parâmetros clínicos e micológicos e grau de satisfação do paciente. Conclusão: apesar da pouca quantidade de ensaios clínicos nesta temática, há evidência de que os produtos naturais podem ser usdos para tratar a candidose oral, especialmente alho, mamona, româ e melaleuca(AU)

Introduction: oral candidiasis is an opportunistic disease that mainly affects immunosuppressed patients and users of total dental prosthesis. Despite the therapeutic efficacy of currently used antifungals for the treatment of this disease, these drugs have many dose related adverse effects. In addition, an increase in microbial resistance has been observed for these agents. Based on this, a promising source of alternative active principles for the treatment of oral candidosis are the natural products. Objective: to review the literature on clinical trials with natural products for the treatment of oral candidosis in order to answer if there is evidence that natural products can be used as an alternative treatment for this disease. Material and method: a synthesis of all clinical trials with natural products for the treatment of candidiasis indexed Pubmed database was performed. Results: following the inclusion and exclusion criteria, 6 studies were included in this review. The studies evaluated the efficacy of the natural products regarding clinical and mycological parameters and degree of patient satisfaction. Conclusion: despite the limited amount of clinical trials on this subject, there is evidence that natural products can be used to treat oral candidosis, especially garlic, castor oil, pomegranate, and melaleuca(AU)

Introducción: la candidose oral es una enfermedad oportunista que afecta principalmente a los pacientes inmunosuprimidos ya los usuarios de prótesis dentales totales. A pesar de la eficacia terapéutica de los antifúngicos utilizados actualmente para el tratamiento de esta enfermedad, estos medicamentos presentan muchos efectos adversos relacionados con la dosis. Además, se ha observado un aumento de la resistencia microbiana para estos agentes. En base a esto, una fuente de búsqueda prometedora de principios activos alternativos para el tratamiento de la candidiasis oral son los productos naturales. Objetivo: revisar la literatura sobre ensayos clínicos con productos naturales para el tratamiento de la candidiasis oral, a fin de responder si existe evidencia que los productos naturales pueden ser utilizados como tratamiento alternativo para esta enfermedad. Material y método: se realizó una síntesis de todos los ensayos clínicos con productos naturales para el tratamiento de la candidose indexada base de datos Pubmed. Resultado: siguiendo los criterios de inclusión y exclusión entraron en esta revisión 6 estudios. Los estudios evaluaron la eficacia de los productos naturales en cuanto a los parámetros clínicos y micológicos y grado de satisfacción del paciente. Conclusión: a pesar de la poca cantidad de ensayos clínicos en esta temática, hay evidencia de que los productos naturales pueden ser utilizados para tratar la candidose oral, especialmente ajo, ricino, granada y melaleuca(AU)

Análise in vitro da desinfecção de escovas dentais por substâncias com potencial antimicrobiano / In vitro analysis of toothbrushes' disinfection by antimicrobial activity substances / Análisis in vitro de la desinfección de cepillos dentales por sustancias con potencial antimicrobiano

Introdução: a correta utilização de técnicas e materiais de higiene são alguns dos fatores que influenciam positivamente a saúde bucal. O mal acondicionamento e higienização das escovas dentais podem contribuir para a reincidência de infecções bucofaríngeas. Objetivo: avaliar, in vitro, o potencial de descontaminação de duas diluições de hipoclorito de sódio em escovas dentais. Materiais e Métodos: as escovas foram inoculadas com Escherichia coli, Candida albicans, Streptococcus mutans e Pseudomonas aeruginosa, durante 30 segundos. Posteriormente foram tratadas por 10 minutos com: solução bucal Colgate Periogard® com álcool e 0,12% de gluconato de clorexidina, Água Sanitária Brilux® com 2,5% de cloro ativo diluída em água destilada até a concentração de 1% e Água Sanitária Brilux® com 2,5% de cloro ativo diluída em água destilada de acordo com a indicação do fabricante para desinfecção de frutas e vegetais (0,005%). Alíquotas das soluções foram semeadas em BHI ágar e incubadas a 37ºC, por 48 horas. Foi feita a contagem das Unidades Formadoras de Colônia UFC/mL. Resultados: para as soluções Colgate Periogard® e Água Sanitária Brilux® (1%) não foi observado crescimento de microrganismos, já a solução Sanitária Brilux® (0,005%) apresentou um discreto crescimento para Streptococcus mutans (10200 UFC/ml) e Candida albicans(1200 UFC/ml). Conclusão: o Colgate Periogard® e a Água Sanitária Brilux® (1%) foram semelhantemente eficazes na desinfecção das escovas. O hipoclorito é uma substância de baixo custo, de fácil acesso e um bom candidato a ser adotado para a indicação de armazenamento e limpeza das escovas(AU)

Introduction: The correct use of techniques and hygiene materials are some of the factors that positively influence oral health. Poorly conditioned and hygienised toothbrushes may contribute to the recurrence of oropharyngeal infections. Objective: to evaluate, in vitro, the decontamination potential of two dilutions of sodium hypochlorite in dental brushes. Materials and Methods: The brushes were inoculated with Escherichia coli, Candida albicans, Streptococcus mutans and Pseudomonas aeruginosa for 30 seconds. They were then treated for 10 minutes with: Colgate Periogard® alcohol solution and 0.12% chlorhexidine gluconate, Brilux® Sanitary Water with 2.5% active chlorine diluted in distilled water to a concentration of 1% and Sanitary Water Brilux® with 2.5% active chlorine diluted in distilled water according to the manufacturer's indication for disinfection of fruits and vegetables (0.005%). Aliquots of the solutions were seeded in BHI agar and incubated at 37 ° C for 48 hours. The UFC / mL colony forming units were counted. Results: Brilux® solution (0,005%) showed a slight growth for Streptococcus mutans (10200 CFU / ml) and Candida albicans (1200 CFU / ml). Conclusion: Colgate Periogard® and Brilux® Sanitary Water (1%) were similarly effective in disinfecting the brushes. Hypochlorite is a low cost, easily accessible substance and a good candidate to be adopted for the storage and cleaning indication of brushes(AU)

Introducción: la correcta utilización de técnicas y materiales de higiene son algunos de los factores que influencian positivamente la salud bucal. El mal acondicionamiento e higienización de los cepillos dentales pueden contribuir a la reincidencia de infecciones bucofaríngeas. Objetivo: evaluar, in vitro, el potencial de descontaminación de dos diluciones de hipoclorito de sodio en cepillos dentales. Materiales y Métodos: los cepillos fueron inoculados con Escherichia coli, Candida albicans, Streptococcus mutans y Pseudomonas aeruginosa, durante 30 segundos. En la mayoría de los casos, se observó un aumento en la concentración de agua potable en el agua potable, con una concentración de 1% y agua sanitaria, Brilux® con 2,5% de cloro activo diluida en agua destilada de acuerdo con la indicación del fabricante para desinfección de frutas y vegetales (0,005%). Las alícuotas de las soluciones fueron sembradas en BHI ágar e incubadas a 37ºC, por 48 horas. Se realizó el conteo de las Unidades Formadoras de Colonia UFC / mL. Resultados: para las soluciones Colgate Periogard® y Agua Sanitaria Brilux® (1%) no se observó crecimiento de microorganismos, ya la solución Sanitaria Brilux® (0,005%) presentó un discreto crecimiento para Streptococcus mutans (10200 UFC / ml) y Candida albicans (1200 UFC / ml). Conclusión: el Colgate Periogard® y el Agua Sanitaria Brilux® (1%) fueron similares en la desinfección de las escobillas. El hipoclorito es una sustancia de bajo costo, de fácil acceso y un buen candidato a ser adoptado para la indicación de almacenamiento y limpieza de los cepillos(AU)

Candida auris: Epidemiology, risk factors, virulence, resistance, and therapeutic options.

Candida auris emerged as a pathogen resistant to multiple antifungal and has been associated with nosocomial outbreaks with high transmission capacity between hospitalized individuals. C. auris was first described in 2009, after being isolated from the external ear canal discharge of a patient in Japan. The difficulty in identification, incorrect use of antifungal drugs, and treatment failure are causes of high mortality. Since then, C. auris has been increasingly reported from East Asia to North America, with substantial fatalities and misidentification. This review aims at describing the epidemiology, virulence, risk factors, resistance, and therapeutic options in C. auris infections.

Experiência de cárie e fatores associados entre escolares das zonas urbana e rural: um estudo piloto / Caries experience and associated factors among urban and rural schoolchildren: a pilot study / Experiencia de caries y factores asociados entre escolares de las zonas urbanas y rurales: un estudio piloto

Introdução: A cárie é uma doença infecciosa, de caráter multifatorial, dependente da dieta, microbiota bucal e resposta do hospedeiro, resultando na desmineralização localizada dos tecidos dentários duros. Objetivo: Avaliar a experiência de cárie e fatores associados em escolares residentes em zonas rurais e urbanas de Araruna, PB. Material e método: Um levantamento epidemiológico de prevalência de cárie dentária e alterações gengivais foi realizado. Os dados foram anotados em fichas odontológicas apropriadas, visando identificar diferenciais entre estes indicadores das duas áreas estudadas. Além disto, o fluxo salivar dos participantes foi determinado. Resultado: Os resultados indicaram diferenças brandas entre os indicadores de saúde bucal nas duas zonas. Apesar da tendência destes indicadores, como o CPOD, ISG e IHOS terem sido mais baixos na zona rural, há alguns anos atrás, do que os observados na zona urbana, no presente trabalho não foram identificadas diferenças significativas entre estes índices. Conclusão: A população rural vem obtendo acesso aos serviços encontrados na cidade, facilitando a inclusão na dieta de alimentos mais cariogênicos e, consequentemente, igualando os índices de saúde bucal aos encontrados na população urbana. Desta forma, o risco de cárie nestas populações, atualmente, é similar(AU)

Introduction: The caries is an infectious, multifactorial disease, dependent on diet, oral microbiota and host response, resulting in localized demineralization of hard dental tissues. Objective: To evaluate the experience of caries and associated factors in schoolchildren residing in rural and urban areas of Araruna, PB. Material and method: An epidemiological survey of the prevalence of dental caries and gingival alterations was performed. The data were annotated in appropriate dental records, in order to identify differences between these indicators of the two areas studied. In addition, the participants' salivary flow was determined. Result: The results indicated mild differences between the oral health indicators in the two areas. Despite the tendency of these indicators, such as CPOD, ISG and IHOS to be lower in rural areas, a few years ago, in the present study, no significant differences were found between these indices. Conclusion: The rural population has been gaining access to the services found in the city, facilitating the inclusion of more cariogenic foods in the diet and, consequently, matching the oral health indexes to those found in the urban population. Thus, the risk of caries in these populations is currently similar(AU)

Introducción: La caries es una enfermedad infecciosa, de carácter multifactorial, dependiente de la dieta, microbiota bucal y respuesta del huésped, resultando en la desmineralización localizada de los tejidos dorsales duros. Objetivo: Evaluar la experiencia de caries y factores asociados en escolares residentes en zonas rurales y urbanas de Araruna, PB. Material y método: Se realizó un levantamiento epidemiológico de prevalencia de caries y cambios en las encías. Los datos fueron anotados en fichas odontológicas apropiadas, buscando identificar diferenciales entre estos indicadores de las dos áreas estudiadas. Además, el flujo salivar de los participantes fue determinado. Resultado: Los resultados indicaron diferencias blandas entre los indicadores de salud bucal en las dos zonas. A pesar de la tendencia de estos indicadores, como el CPOD, ISG e IHOS han sido más bajos en la zona rural, hace algunos años, que los observados en la zona urbana, en el presente trabajo no se identificaron diferencias significativas entre estos índices. Conclusión: La población rural viene obteniendo acceso a los servicios encontrados en la ciudad, facilitando la inclusión en la dieta de alimentos más cariogénicos y, consecuentemente, igualando los índices de salud bucal a los encontrados en la población urbana. De esta forma, el riesgo de caries en estas poblaciones, actualmente, es similar(AU)

Prevalência de erosão dentária e fatores associados em uma população de escolares / Prevalence of dental erosion and associated factors in a schoolchildren population

Introdução: Erosão dentária é a perda irreversível de tecido dentário, sem envolvimento bacteriano, cujo aumento de prevalência, nos últimos anos, tem sido alarmante. Objetivo: Determinar a prevalência de erosão dentária, bem como fatores associados, em escolares de 06 a 13 anos, na cidade de Campina Grande, Paraíba. Material e método: A seleção das escolas foi realizada por amostragem casual simples sem reposição. Após assinatura do Termo de Consentimento Livre e Esclarecido, e do Termo de Assentimento, os exames foram realizados por um examinador previamente calibrado, com a criança sentada em uma cadeira escolar, em frente ao examinador, sob luz natural. Para a determinação da presença e do grau de erosão dentária, foi utilizado o índice de BEWE. Os dados foram analisados por meio do software Statistical Package for the Social Sciences (SPSS para Windows, versão 18.0, SPSS Inc, Chicago, IL, EUA) e adotou-se nível de significância de 5%. Resultado: A prevalência de erosão dentária na população avaliada foi de 78,2%. Não foi possível associar a presença das lesões com os fatores socioeconômicos e dietéticos. Apenas a variável Aplicação Tópica de Flúor (ATF) apresentou relação estatisticamente significativa (p=0,012). Conclusão: A alta prevalência de erosão dentária na população avaliada demonstra a necessidade da implementação de medidas assistenciais e preventivas, em curto e longo prazo.

Introduction: Dental erosion is the irreversible loss of dental tissue, without bacterial involvement, whose increased prevalence in recent years has been alarming. Objective: To determine the prevalence of dental erosion, as well as associated factors, in school children from 06 to 13 years of age, in the city of Campina Grande, PB. Material and method: Selection of schools was performed by simple random sampling without replacement. After signing the Informed Consent Term and Assent Term, the examinations were performed by a previously calibrated examiner, with the child sitting in a school chair in front of the examiner in natural light. To determine the presence and degree of dental erosion, the BEWE index was used. Data were analyzed using Statistical Package for Social Sciences (SPSS for Windows, version 18.0, SPSS Inc, Chicago, IL, USA) and a significance level of 5% was used. Result: The prevalence of dental erosion in the evaluated population was 78.2%. It was not possible to associate the presence of lesions with socioeconomic and dietary factors. Only the variable Topical Application of Fluoride (TFA) presented a statistically significant relation (p = 0.012). Conclusion: The high prevalence of dental erosion in the evaluated population demonstrates the need to implement preventive and preventive measures in the short and long term.